Separation of 21 PTC-amino acids by reverse-phase C18 chromatography

Derivatized amino acids were separated using two mobile phases :

  • solvent A : aqueous sodium acetate 20 mM, trifluoroacetic acid and triethylamine 0.04%
  • solvent B : acetonitrile
  • flow rate : 1.0 mL/min

The PITC reagent had minor peaks (at 15.9, 17.8, 19.2, 21.4, 26.4 and 27.7 min) that did not interfere with the separation of PTC-amino acids.

Although PTC-Cys was detected as standard, it was not detected in the biological samples.

time (min)
% A
% B
increase of B
0
93
7
--------
10
91
9
non linear
13
78
22
linear
23
68
32
non linear
26
20
80
non linear
29
93
7
non linear