Amino acids analysis of axis and cotyledons of Pisum sativum by high performance liquid chromatography

M.-T. Riou & E. Jaspard

 

I. Methods
  • Derivatization of individual amino acids with phenylisothicyanate (PITC)
  • Extraction of amino acids from cotyledons and axis and derivatization with PITC
  • HPLC system


II. Separation of the phenylthiocarbamyl (PTC) amino acids
 

III. Results (standard error bars in the figures correspond to n = 5 to 11 different injections)
 

IV. Global analysis of all individual amino acids

 

IV. Conclusion

V. References