Amino acids analysis of axis and cotyledons of Pisum sativum by high performance liquid chromatography

M.-T. Riou & E. Jaspard


I. Methods
  • Derivatization of individual amino acids with phenylisothicyanate (PITC)
  • Extraction of amino acids from cotyledons and axis and derivatization with PITC
  • HPLC system

II. Separation of the phenylthiocarbamyl (PTC) amino acids

III. Results (standard error bars in the figures correspond to n = 5 to 11 different injections)

IV. Global analysis of all individual amino acids


IV. Conclusion

V. References