Derivatized amino acids were separated using two mobile phases :
- solvent A : aqueous sodium acetate 20 mM, trifluoroacetic acid and triethylamine 0.04%
- solvent B : acetonitrile
- flow rate : 1.0 mL/min
The PITC reagent had minor peaks (at 15.9, 17.8, 19.2, 21.4, 26.4 and 27.7 min) that did not interfere with the separation of PTC-amino acids.
Although PTC-Cys was detected as standard, it was not detected in the biological samples.
